volume 12 number 1 pages 75-75 edition 37929
Forensic Science International
Key Laboratory of Conservation Genetics and Reproductive Biology for Endangered Wild Animals, Ministry of Education, Zhejiang, PR ChinaState Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang University, Zhejiang, PR China
tiger; DNA; forensic; genetics
Qiu-Hong, W., and S. Fang. 2003. Application of species-specific polymerase chain reaction in the forensic identification of tiger species. Forensic Science International 12(1):75. Globally, tigers are considered to be endangered, and are listed on Appendix I of CITES. A simple test, using a species-specific primer pair, was developed to identify tiger meat, faeces and dried skin, and provide forensic evidence of illegal wildlife trade. The specific fragment of mitochondrial cytochrome b gene was also successfully amplified from raw DNA products extracted from single tiger hairs. This PCR-based approach opens a new avenue to forensic identification of less-than-optimal samples.