Application of species-specific polymerase chain reaction in the forensic identification of tiger species.

Authors: 
Qiu-Hong, W. and S. Fang
Document Type: 
peer review publication
Citation: 
volume 12 number 1 pages 75-75 edition 37929
Ref ID: 
6153
Ref User: 
admin
Ref Type: 
Journal Article
Year Published: 
2003
Sec Title: 
Forensic Science International
Isbn Issn: 
0379-0738
Date Input: 
10/30/2006 3:00 AM
Availability: 
PDF upload
Address: 
Key Laboratory of Conservation Genetics and Reproductive Biology for Endangered Wild Animals, Ministry of Education, Zhejiang, PR ChinaState Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang University, Zhejiang, PR China
Language: 
English
Date Modified: 
11/01/2006 3:00
Modified by: 
admin
Last Post: 
11/01/2006 3:00
Bushmeat Asian Type: 
wildlife trade
Keywords: 
tiger; DNA; forensic; genetics
Abstract: 
Qiu-Hong, W., and S. Fang. 2003. Application of species-specific polymerase chain reaction in the forensic identification of tiger species. Forensic Science International 12(1):75. Globally, tigers are considered to be endangered, and are listed on Appendix I of CITES. A simple test, using a species-specific primer pair, was developed to identify tiger meat, faeces and dried skin, and provide forensic evidence of illegal wildlife trade. The specific fragment of mitochondrial cytochrome b gene was also successfully amplified from raw DNA products extracted from single tiger hairs. This PCR-based approach opens a new avenue to forensic identification of less-than-optimal samples.
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